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Integrin alpha 4 beta 7, ITGA4&ITGB7
Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-C52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # XP_005573683.2 (ITGA4) & XP_005571028.2 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.6 kDa (ITGA4) & 81.6 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 60-70 kDa, 95-120 kDa and 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Less than 0.01 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.