Human Integrin alpha L beta 2 (ITGAL&ITGB2) Heterodimer Protein, His Tag&Tag Free

Synonyms

Integrin alpha L beta 2, ITGAL&ITGB2

Source

Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H53W3) is expressed from CHO cells. It contains AA Tyr 26 - Met 1089 (ITGAL) & Gln 23 - Asn 700 (ITGB2) (Accession # P20701-1 (ITGAL) & P05107-1 (ITGB2) (H354Q)).

Predicted N-terminus: Tyr 26 (ITGAL) & Gln 23 (ITGB2)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAL and ITGB2, has a calculated MW of 124.1 kDa (ITGAL) & 80.2 kDa (ITGB2). Subunit ITGAL is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGAL) and 90-95 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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