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Integrin alpha V beta 1, ITGAV&ITGB1
Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (IT1-H5213) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.3 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV contains no tag but an acidic tail at the C-terminus and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGAV) and 100-115 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.