Human KIR2DL3 / CD158b2 / NKAT-2 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
KI3-H5258-100ug
$360.00
KI3-H5258-1mg
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD158b2

  • Source

    Human KIR2DL3, Fc Tag (KI3-H5258) is expressed from human 293 cells (HEK293). It contains AA His 22 - His 245 (Accession # P43628-1).

    Predicted N-terminus: His 22

    Request for sequence
  • Molecular Characterization

    KIR2DL3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.0 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    KIR2DL3 SDS-PAGE

    Human KIR2DL3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    KIR2DL3 SEC-MALS

    The purity of Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) is more than 90% and the molecular weight of this protein is around 108-132 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     KIR2DL3 ELISA

    Immobilized Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) at 1 μg/mL (100 μL/well) can bind Lirilummab with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     KIR2DL3 SPR

    Biotinylated Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) with an affinity constant of 2.40 μM as determined in a SPR assay (Biacore 8K) (Routinely tested)

    Protocol

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Background

Killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) is also known as CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2, p58 natural killer cell receptor clone CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2 and KIR2DL3, which is a single-pass type I membrane protein and belongs to the immunoglobulin superfamily.KIR2DL3 is a receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). KIR2DL3 can inhibit the activity of NK cells thus preventing cell lysis.

Recent Advances

 
Drug Development Progress
  • English Name:

    Killer cell immunoglobulin-like receptor 2DL3

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    1 Details

  • Highest Development Stage:

    Phase 2 Clinical

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