Mouse Klotho beta / KLB Protein, His Tag

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製品番号/仕様
価格
数量
KLB-M52H7-100ug
$395.00
KLB-M52H7-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    betaKlotho, beta-klotho, BKL, KLB, klotho beta like, klotho beta-like protein

  • Source

    Mouse Klotho beta, His Tag (KLB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 995 (Accession # Q99N32-1).

    Predicted N-terminus: Phe 53

    Request for sequence
  • Molecular Characterization

    Klotho beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 110.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Klotho beta SDS-PAGE

    Mouse Klotho beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-BLI

     Klotho beta BLI

    Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 78.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol
  •  Klotho beta BLI

    Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 54.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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バックグランド

KLB (Klotho Beta) is a Protein Coding gene. Among its related pathways are RET signaling and HIV Life Cycle. GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and fibroblast growth factor binding. An important paralog of this gene is KL. Klotho Beta is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased expression of βKlotho compared with normal hepatocyte cells. Reintroduction of βKlotho into hepatoma cells inhibited their proliferation.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Klotho beta

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    3 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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