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製品詳細
Product Details
Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.
Expression System
HEK293
Key Parameter
Purity (SDS PAGE)> 90%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.01 EU per μgFormulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution;
- 2-8°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
Bioactivity-ELISA
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with a linear range of 0.2-20 ng/mL (QC tested).
Protocol
Immobilized Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) immobilized on CM5 Chip can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with an affinity constant of 49.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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