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製品詳細
Product Details
Laminin 411 is a key component of the basement membrane and is involved in the regulation of cell differentiation. Laminin 411 fragment consists of the C-terminal end of the alpha 4, beta 1, and gamma 1 chains.
Expression System
HEK293
Key Parameter
Purity (SDS PAGE)> 90%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.1 EU per μgFormulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution;
- 2-8°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
Bioactivity-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 29.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 17.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human MCAM, His Tag (Cat. No. CD6-H5229) immobilized on CM5 Chip can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with an affinity constant of 0.115 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human MCAM, His Tag (Cat. No. CD6-H5229) captured on CM5 chip via anti-His antibody can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with an affinity constant of 27.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) captured on Protein A Chip can bind Human MCAM, His Tag (Cat. No. CD6-H5229) with an affinity constant of 107 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 26.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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