Mouse Legumain Protein, His Tag (Pro-form, HPLC verified)

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製品番号/仕様
価格
数量
LEN-M52H3-100ug
$350.00
LEN-M52H3-1mg
$2500.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    Legumain

  • Source

    Mouse Legumain Protein, His Tag (LEN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 18 - Tyr 435 (Accession # O89017-1).

    Predicted N-terminus: Val 18

    Request for sequence
  • Molecular Characterization

    Legumain Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 49.4 kDa. The protein migrates as 43-48 kDa and 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 20 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Legumain SDS-PAGE

    Mouse Legumain Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    Legumain SEC-HPLC

    The purity of Mouse Legumain Protein, His Tag (Cat. No. LEN-M52H3) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity

    Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >350 pmol/min/μg (QC tested).

    Protocol

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バックグランド

This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis.

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