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製品詳細
Synonyms
LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
Source
Cynomolgus LGR5, His Tag (LG5-C52H7) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 557 (Accession # G7PI19-1).
Predicted N-terminus: Ser 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Cynomolgus LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Cynomolgus LGR5, His Tag (Cat. No. LG5-C52H7) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) at 5 μg/mL (100 μL/well) can bind Cynomolgus LGR5, His Tag (Cat. No. LG5-C52H7) with a linear range of 0.1-10 ng/mL (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Leucine-rich repeat-containing G-protein coupled receptor 5
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
2 詳細
- 最新の開発ステージ：
Phase 2 Clinical
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