Cynomolgus MASP2 Protein, His Tag

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Cat. No. / Size
Price
Qty
MA2-C51H3-100ug
$350.00
MA2-C51H3-1mg
$2160.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    MASP2, MASP-2, Mannan-binding lectin serine protease 2, MASP1P1, sMAP

  • Source

    Cynomolgus MASP2 Protein, His Tag (MA2-C51H3) is expressed from E. coli cells. It contains AA Thr 287 - Phe 686 (Accession # A0A2K5UJY0).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    MASP2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.6 kDa. The protein migrates as 42-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 200 mM NaCl, 0.2 M Arginine, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    MASP2 SDS-PAGE

    Cynomolgus MASP2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     MASP2 ELISA

    Immobilized Cynomolgus MASP2 Protein, His Tag (Cat. No. MA2-C51H3) at 1 μg/mL (100 μL/well) can bind Anti-MASP2 Reference Antibody, Human IgG4 lambda with a linear range of 0.1-3 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     MASP2 SPR

    Narsoplimab captured on Protein A Chip can bind Cynomolgus MASP2 Protein, His Tag (Cat. No. MA2-C51H3) with an affinity constant of 38.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Mannan-binding lectin-associated serine protease 2 (MASP-2) is a member of the lectin pathway of complement and is one of two splice products from the MASP2 gene. The protein is secreted from the liver as a zymogenic protein consisting of 671 residues and circulates at a concentration of approximately 0.41 μg/mL in the serum. MASP-2 is associated with pattern-recognition molecules as a homodimer that is activated by MASP-1 when bound to an activating surface resulting in an A-chain and a B-chain linked by a disulphide bond. MASP-2 is also capable of autoactivating. Activated MASP-2 cleaves C2 and C4, which leads to formation of the C3 convertase, C4b2a, and activation of complement.

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