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Product Details
Synonyms
MCAM, CD146, MUC18
Source
Human MCAM, His Tag (CD6-H5229) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Gly 559 (Accession # NP_006491.2).
Predicted N-terminus: Val 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.5 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human MCAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-SPR
Human MCAM, His Tag (Cat. No. CD6-H5229) immobilized on CM5 Chip can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with an affinity constant of 0.115 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) captured on Protein A Chip can bind Human MCAM, His Tag (Cat. No. CD6-H5229) with an affinity constant of 107 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.
As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition.
Recent Advances
Drug Development Progress- English Name：
Melanoma cell adhesion molecule
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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