Human MICA Protein, Fc Tag

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Cat. No. / Size
Price
Qty
MIA-H5253-100ug
$395.00
MIA-H5253-1mg
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    MIC-A

  • Source

    Human MICA, Fc Tag (MIA-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1).

    Predicted N-terminus: Glu 24

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  • Molecular Characterization

    MICA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 59.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    MICA SDS-PAGE

    Human MICA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     MICA ELISA

    Immobilized Human NKG2D, His Tag (Cat. No. NKD-H5245) at 2 μg/mL (100 μL/well) can bind Human MICA, Fc Tag (Cat. No. MIA-H5253) with a linear range of 0.4-2 ng/mL (QC tested).

    Protocol
  •  MICA ELISA

    Immobilized Human MICA, Fc Tag (Cat. No. MIA-H5253) at 1 μg/mL (100 μL/well) can bind Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) with a linear range of 0.6-20 ng/mL (Routinely tested).

    Protocol

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Background

MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.

Recent Advances

 
Drug Development Progress
  • English Name:

    MHC class I polypeptide-related sequence A

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    4 Details

  • Highest Development Stage:

    Phase 1 Clinical

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