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Product Details
Synonyms
NRP1, Neuropilin-1, NRP, VEGF165R, CD304
Source
Mouse Neuropilin-1, His Tag (NR1-M52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Pro 856 (Accession # P97333-1).
Predicted N-terminus: Phe 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 95.5 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse Neuropilin-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse Neuropilin-1 Protein, His Tag (Cat. No. NR1-M52H3) with a linear range of 2-20 ng/mL (QC tested).
Protocol
Immobilized Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) at 5 μg/mL (100 μL/well) can bind Mouse Neuropilin-1, His Tag (Cat. No. NR1-M52H3) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) immobilized on CM5 Chip can bind Mouse Neuropilin-1, His Tag (Cat. No. NR1-M52H3) with an affinity constant of 4.48 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Neuropilin-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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