GENIUS™Nuclease

Product Details

GENIUS™Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACROBiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

Features and Advantages

1. Generally usable: Generally usable for removing all forms of DNA and RNA from biological products, reducing viscosity and preventing cell clumping.
2. Efficient: Efficient reaction, rapid degradation, reduce processing time.
3. Native: Native structure, tag free, the enzyme activity is more guaranteed.
4. Ideal: Ideal for DNA and RNA clearance
5. Ultra pure: Ultra pure: High purity and activity
6. Specific: Specific nuclease, no protease activity

Application

Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological applications: removal of nucleic acid from protein samples ( Elimination of nucleic acids from recombinant proteins; Purification of protein fragments from inclusion bodies; Sample preparation in western blotting or two- dimensional gel electrophoresis) ; Viscosity reduction in protein extracts.

Operating Conditions

GENIUS™Nuclease is functional between pH 6 and 10 (optimal at pH8 - 8.5) , and from 0℃ to 42 ℃ (optimal at 35 ℃ - 42 ℃). Mg2+ (1-2 mM) is required for enzyme activity. 1 mM EDTA reduced the activity by 30% in the presence of 1 mM MgCl2; 0.1 M EDTA eliminated all enzyme activity. In the presence of 1 mM MgCl2, enzyme levels were reduced 75% by 0.1 M CaCl2 or 1 M NaCl. Under standard assay conditions, 1 mM iodoacetate had no effect on the enzymatic rate, whereas 1 mM mercaptoethanol and maleic acid reduced the activity by only 5 to 10%. 10 mM p- Chloromercurybenzoate completely inactivates the enzyme, while 0.64 M beta-mercaptoethanol in the presence of 2 M urea causes only partial inactivation of the enzyme. 4 or 7 M Urea increases the enzyme activity.

Unit Definition

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.

Purity

>95% as determined by SDS-PAGE.

Enzyme Activity

>250 U/μL

Sterility

Negative

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl, 1 mM MgCl2, pH 8.0.

Contact us for customized product form or formulation.

Shipping and Storage

This product is shipped at ambient temperature.

This product is stable after storage at:

1. In lyophilized state for 1 year (-20℃); After reconstitution under sterile conditions for 3 months (-70℃).