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製品詳細
Synonyms
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
Source
Hamster PCSK9, His Tag (PC9-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 691 (Accession # G3GTK5).
Predicted N-terminus: Gln 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.0 kDa respectively. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Hamster PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with a linear range of 0.078-0.313 μg/mL (QC tested).
Protocol
Immobilized Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) at 1 μg/mL (100 μL/well) can bind Evolocumab with a linear range of 2-63 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with an affinity constant of 4.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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よくある質問
- Product
What key experimental conditions should be considered for PCSK9–LDLR binding assays?
It is recommended to perform PCSK9–LDLR binding assays under acidic conditions, as the interaction exhibits higher activity in acidic environments compared to neutral ones. Reference: Effects of pH and Low Density Lipoprotein (LDL) on PCSK9-dependent LDL Receptor Regulation.
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最新の進展
医薬品開発の進捗- 英語名：
Proprotein convertase subtilisin kexin type 9
- カテゴリ：
- 市販の医薬品数：
8 詳細
- 治験医薬品数：
35 詳細
- 最新の開発ステージ：
Approved
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