Product Details
Synonyms
HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs
Source
Human PRMT5 & MEP50 Protein, Flag,His Tag (PR5-H55D3) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Leu 637 & Arg 2 - Glu 342 (Accession # O14744-1 & Q9BQA1).
Predicted N-terminus: Met & Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
The PRMT5 & MEP50 protein carries a Flag tag and a polyhistidine tag at the N-terminus. The protein has a calculated MW of 74.4 kDa & 38.3 kDa. The protein migrates as 40-43 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE

Human PRMT5 & MEP50 Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human PRMT5 & MEP50 Protein, Flag,His Tag (Cat. No. PR5-H55D3) is more than 90% and the molecular weight of this protein is around 410-460 kDa verified by SEC-MALS.
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