Human PRMT5 & MEP50 Protein, Flag,His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
PR5-H55D3-100ug
$350.00
PR5-H55D3-1mg
$2310.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs

  • Source

    Human PRMT5 & MEP50 Protein, Flag,His Tag (PR5-H55D3) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Leu 637 & Arg 2 - Glu 342 (Accession # O14744-1 & Q9BQA1).

    Predicted N-terminus: Met & Met

    Request for sequence
  • Molecular Characterization

    PRMT5 & MEP50 Structure

    Other Tags and Version Biotin & Other Labeled Version

    The PRMT5 & MEP50 protein carries a Flag tag and a polyhistidine tag at the N-terminus. The protein has a calculated MW of 74.4 kDa & 38.3 kDa. The protein migrates as 40-43 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a heterodimer.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    PRMT5 & MEP50 SDS-PAGE

    Human PRMT5 & MEP50 Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PRMT5 & MEP50 SEC-MALS

    The purity of Human PRMT5 & MEP50 Protein, Flag,His Tag (Cat. No. PR5-H55D3) is more than 90% and the molecular weight of this protein is around 410-460 kDa verified by SEC-MALS.

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Background

Protein arginine methyltransferase 5 (PRMT5) is a type II enzyme that catalyzes symmetric dimethylation of arginine residues on histone and non-histone proteins. It forms a hetero-octameric complex with its essential cofactor MEP50 (methylosome protein 50), which enhances its catalytic activity and substrate selectivity. The PRMT5–MEP50 complex regulates gene expression epigenetically, influences RNA splicing, and modulates signal transduction pathways. Aberrant PRMT5–MEP50 activity is linked to tumorigenesis, cardiovascular disorders, and neurodegeneration by driving dysregulated methylation patterns that favor cell proliferation and survival.

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