Human PSCA Protein, His Tag

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製品番号/仕様
価格
数量
PSA-H52H6-100ug
$470.00
PSA-H52H6-1mg
$3400.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    PSCA, UNQ206, PRO232

  • Source

    Human PSCA, His Tag (PSA-H52H6) is expressed from human 293 cells (HEK293). It contains AA Leu 12 - Ser 86 (Accession # O43653-1).

    Request for sequence
  • Molecular Characterization

    PSCA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 10.4 kDa. The protein migrates as 17-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    PSCA SDS-PAGE

    Human PSCA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     PSCA ELISA

    Immobilized Human PSCA, His Tag (Cat. No. PSA-H52H6) at 1 μg/mL (100 μL/well) can bind Anti-PSCA Antibody,Human IgG1 with a linear range of 0.2-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     PSCA FACS

    2e5 of anti-PSCA CAR-293 cells were stained with 100 μL of 3 μg/mL of Human PSCA, His Tag (Cat. No. PSA-H52H6) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).

    Protocol

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バックグランド

The Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI)-anchored protein, plays an important role in tumorigenesis. The prostate stem cell antigen (PSCA) gene, which encodes a prostate-specific antigen (PSA), was identified as a gene involved in cell adhesion and proliferation. PSCA may be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Prostate stem cell antigen

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    4 詳細

  • 最新の開発ステージ:

    Phase 1 Clinical

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