Human SHP-2 Protein, His Tag

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製品番号/仕様
価格
数量
SH2-H5248-100ug
$390.00
SH2-H5248-1mg (500ug X 2)
$3500.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    SHP-2

  • Source

    Human SHP-2, His Tag (SH2-H5248) is expressed from E. coli cells. It contains AA Thr 2 - Arg 593 (Accession # Q06124-2).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    SHP-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 69.3 kDa. The protein migrates as 65-66 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    SHP-2 SDS-PAGE

    Human SHP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity

    Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is >3 umol/min/mg, measured under the described conditions (QC tested).

    Protocol
  •  SHP-2 ENZYME

    Serial dilutions of SHP394 (an orally efficacious inhibitor of protein tyrosine phosphatase SHP2) were added into Human SHP-2 Protein, His Tag(SH2-H5248) enzymatic reactions. The half maximal inhibitory concentration (IC50) is 19.36 nM (Routinely tested).

    Protocol

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バックグランド

SHP-2, a cytoplasmic SH2 domain containing protein tyrosine phosphatase, is involved in the signaling pathways of a variety of growth factors and cytokines. SHP-2 takes key role in transducing signal relay from the cell surface to the nucleus, and is a important intracellular regulator in mediating cell proliferation and differentiation.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Src homology phosphotyrosyl phosphatase 2

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    21 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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