お気に入りに追加
製品詳細
Synonyms
CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15
Source
Human Siglec-15, His Tag (SG5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
Predicted N-terminus: Phe 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.4 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl, 0.5 M Arginine, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
お客様はこちらも閲覧
データ表示
SDS-PAGE
Human Siglec-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human Siglec-15, His Tag (Cat. No. SG5-H52H3) is more than 85% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 1 μg/mL (100 μL/well) can bind Mouse Human chimeric mAb (5G12, Human IgG1) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 10 μg/mL (100 μL/well) can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-0.313 μg/mL (Routinely tested).
Protocol
ユーザーレビュー コメントを投稿
1***22019-10-11
いいねSiglec-15 is a relatively new type of immune checkpoint molecule. In the process of antibody molecule screening, we used ACRO's Siglec-15 antigen. It has shown very good stability in protein-level binding experiments, and the bioactivity was also quite good which was verified in functional experiments.- 1***32019-10-11 いいねThe product is pretty good and the price is competitive. Besides, the delivery was fast. In particular, there is a literature introduction cited the product. It is obvious that each target has been carefully studied.
よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
バックグランド
最新の進展
医薬品開発の進捗- 英語名：
Sialic acid-binding Ig-like lectin 15
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
3 詳細
- 最新の開発ステージ：
Phase 2 Clinical
お問い合わせ
メールアドレス
order.jp@acrobiosystems.com
お問い合わせ
グローバル：+1 800-810-0816(Toll Free)
医薬品候補ライセンス
