Mouse Siglec-3 / CD33 Protein, His Tag (MALS verified)

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製品番号/仕様
価格
数量
CD3-M52H4-100ug
$360.00
CD3-M52H4-1mg
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD33, SIGLEC3, gp67

  • Source

    Mouse Siglec-3, His Tag (CD3-M52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - Glu 240 (Accession # Q63994-1).

    Predicted N-terminus: Asp 18

    Request for sequence
  • Molecular Characterization

    Siglec-3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Siglec-3 SDS-PAGE

    Mouse Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    Siglec-3 SEC-MALS

    The purity of Mouse Siglec-3, His Tag (Cat. No. CD3-M52H4) is more than 90% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Siglec-3 ELISA

    Immobilized Mouse Siglec-3, His Tag (Cat. No. CD3-M52H4) at 1 μg/mL (100 μL/well) can bind Anti-CD33 antibody, Mouse IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

    Protocol

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バックグランド

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cD encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Myeloid cell surface antigen CD33

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    44 詳細

  • 最新の開発ステージ:

    Approved

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