Human SOD1 / Cu-Zn SOD Pre-formed Fibrils Protein, His Tag

SOD1, Superoxide dismutase 1, hSod1

Human SOD1 Pre-formed Fibrils Protein, His Tag (SO1-H5146) is expressed from E. coli cells. It contains AA Ala 2 - Gln 154 (Accession # P00441).

Predicted N-terminus: Met

1. Sonication Conditions, Dissolution, Aliquoting, Storage, and Notes for PFF

Thawing: Thaw PFFs rapidly in a 37 °C water bath, or allow to thaw at room temperature.

Aliquoting: Since PFFs are supplied as a suspension, pipette up and down thoroughly before aliquoting to ensure homogeneity.

Storage: Store at -80 °C at all times; avoid storage at 4 °C or -20 °C, which can induce fibril depolymerization. * α-Syn fibrils cold-denatured to monomers at 0-20 °C and heat-denatured at 60-110 °C.

Sonication: The sonication protocols below are based on cell-based assay conditions.

-Option 1 (Probe Sonicator): Use a probe sonicator (SCIENTZ) at 10% power (-95 W), applying 60 pulses of 0.5 s on/0.5 s off (recommended).

-Option 2 (Ultrasonic Bath): Sonicate in an ultrasonic cleaner (40 kHz, 200-400 W) at 37 °C for 1 hour; avoid performing the treatment at 4 °C or 20 °C.

Note: The above sonication protocols are based on cell-based assays; other applications (e.g., in vivo injections) may require optimization-users should determine their optimal settings for their specific use case.

2. Recommendations for PFF Use in Animal Models

Minimize Freeze-Thaw Cycles: For animal studies, it is recommend to use the PFF at once or aliquot before use to avoid repeated freeze-thaw cycles.

PFF Maintenance During Injections: During stereotaxic injections, keep the sonicated PFFs in a 37 °C water bath to prevent re-aggregation or sedimentation.

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This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

• The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;
• The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;
• The sonicated fibril should be stored at -70°C for not more than 8 weeks.