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MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified)

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SPN-M52H5-50ug
$465.00
In Stock - Delivery in 2-3 business days
SPN-M52H5-500ug
$2885.00
In Stock - Delivery in 2-3 business days
総アイテム数 製品金額$ 0
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製品詳細

  • Synonyms

    Spike, S protein, Spike glycoprotein, S glycoprotein

  • Source

    MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (SPN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K0BRG7 (R748A, R751A, V1060P, L1061P)). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.

    Predicted N-terminus: Tyr 18

    Request for sequence

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Spike protein SDS-PAGE

    MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    Spike protein SEC-MALS

    The purity of MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (Cat. No. SPN-M52H5) is more than 90% and the molecular weight of this protein is around 540-580 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    Spike protein ELISA

    Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) with a linear range of 0.1-3 ng/mL (QC tested).

    Protocol

  • Spike protein ELISA

    Immobilized MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) at 5 μg/mL (100 μL/well) can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.1-2 ng/mL (Routinely tested).

    Protocol

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  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

The MERS or Middle East Respiratory Syndrome Coronavirus, is a member of the coronavirus family and is known to cause severe respiratory illness in humans. The MERS protein is a type I transmembrane protein that plays a vital role in the virus's ability to infect host cells. It is composed of three main domains: the extracellular domain, the transmembrane domain, and the cytoplasmic domain. The extracellular domain, further divided into S1 and S2 subunits, is responsible for receptor recognition and binding. The S1 subunit contains the Receptor Binding Domain (RBD), which is crucial for the virus's attachment to and entry into host cells. The RBD specifically interacts with the host cell receptor, facilitating the initial step of infection. The S2 subunit, on the other hand, is responsible for membrane fusion. It contains a fusion peptide that inserts into the host cell membrane, initiating the fusion process. This fusion allows the virus's genetic material to be released into the host cell, initiating the infection process.

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医薬品開発の進捗
  • 英語名：

    Coronavirus spike glycoprotein

  • カテゴリ：

  • 市販の医薬品数：

    15 詳細

  • 治験医薬品数：

    88 詳細

  • 最新の開発ステージ：

    Approved

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