SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag

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S1N-C52HD-100ug
$390.00
S1N-C52HD-1mg
$2,875.00
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  • Product Details
  • Performance Data
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  • Important Statement
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  • 分子别名(Synonym)

    Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1

  • 表达区间及表达系统(Source)

    SARS-CoV-2 S1 protein (HV69-70del), His Tag (S1N-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1 (HV69-70del)). HV69-70 deletion was identified in the SARS-CoV-2 Alpha variant (Pango lineage: B.1.1.7; other names: 20I/501Y.V1 or VOC 202012/01).

    Predicted N-terminus: Val 16

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    Spike S1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 76.7 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.