T4 RNA Ligase 1(10U/ul)

T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi.

Joining RNA to RNA
Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Specific modifications of tRNAs
RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
Site-specific generation of composite primers for PCR

One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.

>90% as determined by SDS-PAGE.

The coexistence of PEG promotes the response without changing the specificity of the reaction.

Supplied as 0.2 μm filtered solution in 10 mM Tris, 0.1 mM EDTA, 1 mM DTT, pH 7.4 with glycerol as protectant.

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This product is supplied and shipped on dry ice.

• This product is stable for up to 12 months at -20℃ from date of receipt.