T7 RNA Polymerase (200U/μl)

T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications.

Synthesis of the single-strand RNA
Synthesis of highly labeled RNA probes
Synthesis of precursors of siRNA
Synthesis of precursors for RNA splicing reactions
Synthesis of capped mRNA when a cap analog is used as a primer

One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 37°C.

>95% as determined by SDS-PAGE.

Supplied as 0.2 μm filtered solution in 50 mM Tris, 100 mM NaCl, 1 mM EDTA, 2 mM TCEP, 0.1 % TritonX-100, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

This product is supplied and shipped on dry ice.

• This product is stable for up to 12 months at -20℃ from date of receipt.