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製品詳細
Synonyms
TIGIT, VSIG9, VSTM3
Source
Canine TIGIT, His Tag (TIT-C52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 17 - Pro 208 (Accession # F1PKJ0-1).
Predicted N-terminus: Phe 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Canine TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Canine TIGIT, His Tag (Cat. No. TIT-C52H7) is more than 95% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Canine TIGIT, His Tag (Cat. No. TIT-C52H7) at 5 μg/mL (100 μL/well) can bind Human CD155, mouse IgG2a Fc tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Canine TIGIT, His Tag (Cat. No. TIT-C52H7) with an affinity constant of 5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
T cell immunoglobulin and ITIM domains
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
41 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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