Human TNF-alpha Protein, Research Grade

• Native Conformation: Native sequences, tag-free and natural function.
• Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
• Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
• Lowest Endotoxin level (<0.01 EU/ug).
• Safety Assurance: Sterile filtration through 0.2 μm membrane.
• AOF: Animal origin-free raw materials throughout the production process.

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

Human TNF-alpha Protein, Research Grade (TNA-H5213) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).

Less than 0.01 EU per μg by the LAL method / rFC method.

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 24 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.

ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.