Human TNF-alpha Protein, His Tag, active trimer, low endotoxin (MALS verified)

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

Human TNF-alpha Protein, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Less than 0.01 EU per μg by the LAL method / rFC method.

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 12 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.