Human TNF-beta / Lymphotoxin-alpha Protein, Fc Tag (MALS verified)

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製品番号/仕様
価格
数量
TNB-H5263-100ug
$350.00
TNB-H5263-1mg
$2310.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    LTA, DAMA-25N12, 13-004, LT, TNFB, TNFSF1, LT-alpha, TNF-beta

  • Source

    Human TNF-beta Protein, Fc Tag (TNB-H5263) is expressed from human 293 cells (HEK293). It contains AA Lys 62 - Leu 205 (Accession # P01374).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    TNF-beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 42.3 kDa. The protein migrates as 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a trimer.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    TNF-beta SDS-PAGE

    Human TNF-beta Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TNF-beta SEC-MALS

    The purity of Human TNF-beta Protein, Fc Tag (Cat. No. TNB-H5263) is more than 85% and the molecular weight of this protein is around 255-285 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TNF-beta ELISA

    Immobilized Human TNFR2, His Tag (Cat. No. TN2-H5227) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, Fc Tag (Cat. No. TNB-H5263) with a linear range of 0.2-5 ng/mL (QC tested).

    Protocol
  •  TNF-beta ELISA

    Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, Fc Tag (Cat. No. TNB-H5263) with a linear range of 0.2-5 ng/mL (Routinely tested).

    Protocol

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バックグランド

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Tumor necrosis factor β

  • カテゴリ:

  • 市販の医薬品数:

    18 詳細

  • 治験医薬品数:

    7 詳細

  • 最新の開発ステージ:

    Approved

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