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TCR-CD3 Complex

Tumor immunotherapy and autoimmune disease treatment are key areas in global immune drug development. CD3-targeted T-cell engagers (TCEs) have shown strong potential in both fields by enabling precise T-cell redirection. However, adverse events such as cytokine release syndrome (CRS) remain a major challenge, limiting their therapeutic window and broader clinical utility.

Under physiological conditions, CD3 exists as an integral component of the multi-subunit TCR–CD3 complex on the T-cell surface, mediating antigen recognition and T-cell activation. Screening based on the native TCR-CD3 complex better mimics physiological conditions, enabling more accurate evaluation of antibody binding and function. It also facilitates comprehensive epitope mapping across CD3, helping identify optimal epitopes that balance potent immune activation with reduced CRS risk.

To address this critical need, we offer TCR–CD3 complex proteins that faithfully reproduce the complete assembly and native conformation of TCR and CD3 subunits. This delivers a robust platform for CD3-targeted antibody discovery and evaluation, accelerating the development of safer and more effective next-generation immunotherapies.

Product Features

Native-like Conformation:

Replicates the complete transmembrane complex, delivering true-to-life physiological relevance.

Comprehensive-Validated Performance:

Proven by anti-CD3, anti-TCR αβ antibody and pMHC binding assays, confirming both structural integrity and functional activity.

Multi-scenario Formats:

VLP / Detergent / Nanodisc-pro options available, fully supporting diverse flexible R&D workflows.

TCR-CD3 (VLP)

Recommended Applications: Immunization, Screening, Validation (ELISA/Cell-Based Assays)

TCR-CD3 (Detergent)

Recommended Applications: Screening, Validation (ELISA/SPR)

TCR-CD3 (Nanodisc-pro)

Recommended Applications: Screening, Validation (ELISA/SPR/Cell-Based Assays)

Product List

Cat. No.	Molecule	Product Description	Order Now
NYR-H5258	NY-ESO-1 TCR	Human NY-ESO-1 TCR Protein, Fc Tag (MALS & SPR verified)	Order
NYZ-H5213	NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z	Human NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z Full Length Protein Complex (VLP)	Order
NYZ-H52D3	NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z	Human NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z Full Length Protein Complex, His Tag&Flag Tag (Nanodisc-pro)	Order
NYZ-H52D4	NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z	Human NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z Full Length Protein Complex, His Tag&Flag Tag (Detergent)	Order
TCZ-R5213	TCR & CD3E & CD3D & CD3G & CD3Z	Rhesus macaque TCR & CD3E & CD3D & CD3G & CD3Z Full Length Protein Complex (VLP)	Order

Validation Data

CD3 Domain Validation: Specific binding to multiple anti-CD3 antibodies

CD3 Domain Validation-1

Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.2-3 ng/mL (QC tested).

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CD3 Domain Validation-2

Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind Llama Anti-CD3 with a linear range of 0.05-2 ng/mL (Routinely tested).

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CD3 Domain Validation-3

Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) captured on CM5 chip via anti-mouse antibodies surface can bind Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) with an affinity constant of 2.72 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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TCR Domain Validation: Specific binding to anti-TCR α/β antibody

TCR Domain Validation

Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind FITC anti-human TCR α/β Antibody with a linear range of 0.01-0.625 μg/mL (Routinely tested).

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TCR Functional Validation: Specific binding to pMHC

TCR Domain Validation

Immobilized Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) with a linear range of 0.01-0.313 μg/mL (QC tested).

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