お気に入りに追加
製品詳細
Source
Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
M5D04
Isotype
Rabbit IgG, Kappa
Immunogen
DM-1-BSA
Specificity
Specifically recognizes the target DM-1 & DM-4.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
お客様はこちらも閲覧
データ表示
Bioactivity-ELISA
Immobilized Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) at 5 μg/mL, add DM1 Antibody Human IgG in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) at 1 μg/mL (100 μL/well) can bind DM1 Antibody Human IgG with a linear range of 0.004-0.125 μg/mL (Routinely tested).
Protocol
Serial dilutions of DM1 were added into Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358): DM1 Antibody Human IgG binding reactions. The half maximal inhibitory concentration (IC50) is 3.149 μg/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) with DM1 Antibody Human IgG, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF conjugated antibody respectively.
The coating antibody was Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including DM1 Antibody Human IgG, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) is specific to DM1 Antibody Human IgG, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF (Routinely tested).
ユーザーレビュー コメントを投稿
バックグランド
最新の進展
お問い合わせ
メールアドレス
order.jp@acrobiosystems.com
お問い合わせ
グローバル：+1 800-810-0816(Toll Free)
医薬品候補ライセンス
