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製品詳細
Source
Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) is a recombinant rabbit monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
1M2C3
Isotype
Rabbit IgG, Kappa
Immunogen
Doxorubicin-BSA
Specificity
Specifically recognizes Doxorubicin.
Application
ApplicationRecommended UsageELISA0.2-2 μg/mLPurification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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データ表示
Bioactivity-ELISA
Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) with a linear range of 0.06-8 ng/mL (QC tested).
Protocol
Serial dilutions of Doxorubicin were added into Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216): Doxorubicin-ADC binding reactions. The half maximal inhibitory concentration (IC50) is 0.009186 μg/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) with Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecan and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecan and Trastuzumab-DM1 conjugated antibodies used at 0.25 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) is specific to Doxorubicin-ADC and has no cross-reactivity with IgG1-MMAF, Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecan and Trastuzumab-DM1 (Routinely tested).
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