Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1A03)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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製品番号/仕様
価格
Availability
数量
DUN-MY2288-100ug
$315.00
In Stock - Delivery in 2-3 business days
DUN-MY2288-1mg (500ug X 2)
$1890.00
In Stock - Delivery in 2-3 business days
総アイテム数 製品金額$ 0

製品詳細

  • Source

    Chimeric Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1A03) is recombinantly expressed in HEK293. It consists of rabbit variable regions genetically fused to rabbit IgG constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    M1A03

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Immunogen

    Duocarmycin-BSA

  • Specificity

    Specifically recognizes the Duocarmycin and Duocarmycin structurally similar molecules.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-ELISA

     Duocarmycin ELISA

    Serial dilutions of Duocarmycin were added into BSA-Duocarmycin: Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1A03) (Cat. No. DUN-MY2288) binding reactions. The half maximal inhibitory concentration (IC50) is 0.03 μg/mL (QC tested).

    Protocol

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よくある質問

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

Duocarmycin, a DNA minor groove-binding alkylating agent, acts as a payload in antibody-drug conjugates (ADCs) by selectively targeting AT-rich regions of DNA. It forms covalent bonds with the N3 position of adenine, inducing strand breaks and apoptosis through S-phase cell cycle arrest . This mechanism bypasses multidrug resistance pathways, making it potent against aggressive tumors.

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