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Product Details
- ItemsSize （5mg）Size（5mg X 2）Particle size2 μm2 μmPhysical appearancePowder mixturePowder mixtureFormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% TrehaloseReconstitution5 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
Product Description
The streptavidin magnetic beads are uniform size, narrow size distribution with large surface area and unique surface coating, it is easy to capture the biotinylated proteins or other molecules, and the bounded protein have no activity lost, can help us get the best performance and highly reproducible results.
Application
This product is intended for immunocapture, biopanning. This is a non-sterile product.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable for 1 year when stored at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
Assay Principles
Application Method:
a) Reconstitute the beads with ultrapure water to 1mg/mL, and wash the beads for three times.
b) Dilute the biotinalytated protein or antibodies with Assay Buffer, and add to the beads, incubate for 60 minutes at room temperature in a rotary mixer.
c) Wash the beads for four times, and add assay buffer to beads for next experiment.Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. The Magenetic beads are pre-coupled with Streptavidin tetramer protein and blocked with BSA, it is a ready-to-use beads, which can be used for binding biotinylated proteins and antibodies.
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Performance Data
Typical Data
SA-Beads conjugated with human uPAR protein are used to bind mouse anti-uPAR antibody, with PE anti-mouse IgG1 Fc as detection reagent. EC50 of 0.537 μg/mL (QC-tested).
Protocol
Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, with an EC50 0.1750 μg/mL (Routinely tested).
Protocol
Freeze-thaw stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after different freeze-thaw cycles, add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Three freeze-thaw cycles were performed (Routinely tested).
Protocol
Accelerated stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after placing the lyophilized beads at 37°C for 7 daysadd sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Data were measured on day 0, 3, 7 respectively (Routinely tested).
Protocol
Customer Reviews Writing Reviews
L***u2023-05-24
LikesStreptavidin coated magnetic beads (cat# SMB-B01) lot # SMB1-2299-15Z was used as immunocapture to remove the biotinylated proteins in the samples, and the supernatant containing target proteins in the samples were proceeded for the next step of ELISA. The SA magnetic beads are convenient and are easily to handle. The result showed the biotinylated proteins were significantly removed after the incubation with SA-beads, and it demonstrated the SA beads performed very well. Additional to this experiment, the SA beads will be considered for other applications because of the good performance.
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