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製品詳細
Synonyms
ACVR2B, ACTRIIB, MGC116908
Source
Biotinylated Mouse Activin RIIB Protein, His,Avitag (ACB-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Thr 137 (Accession # P27040-1).
Predicted N-terminus: Ser 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.3 kDa. The protein migrates as 25-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Biotinylated Mouse Activin RIIB Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Mouse Activin RIIB Protein, His,Avitag (Cat. No. ACB-M82E3) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Latent Activin A, His Tag (Cat. No. ACA-H424x) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse Activin RIIB Protein, His,Avitag (Cat. No. ACB-M82E3) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Mouse Activin RIIB Protein, His,Avitag (Cat. No. ACB-M82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human Activin A Protein, Tag Free (Cat. No. ACA-H421b) with an affinity constant of 0.106 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
最新の進展
医薬品開発の進捗- 英語名：
Activin receptor type-2B
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
5 詳細
- 最新の開発ステージ：
Phase 2 Clinical
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