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Streptavidin(SA) Series Products

In 1963, Stapley EO first isolated streptavidin (SA) from the culture medium of Streptomyces avidinii. The binding between SA and biotin is one of the strongest non-covalent interactions known in nature. SA and biotin bind with high affinity producing a significant amplification effect, making the SA-biotin system an asset that is widely utilized in biological and chemical experiments.
SA-biotin systems are often coupled to antibodies or proteins, where SA is conjugated to labels such as HRP (Horseradish Peroxidase), fluorophores, etc., and biotin is coupled to antibodies or proteins (MABSOL® Biotinylated Proteins). The SA-biotin system is widely used in the qualitative and quantitative detection of trace antigens, antibodies and localization observation research to achieve indirect detection of targets, making immunolabeling and related tracer analysis more sensitive.
ACROBiosystem’s SA series of products includes recombinant SA protein, labeled SA protein, SA coated plates, SA magnetic beads, etc., to accelerate your scientific research projects and drug development. Among them, the labeled SA protein contains premium grade PE labeled SA protein, which are strictly sterile and can be used for preclinical research in cell-related experiments.

SA Series Products

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Verification Data

Application data for PE labeled SA protein(FACS)

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Application data for SA(ELISA)

STN-N5116-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

STN-N5116-E2

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Application data for SA-HRP

Application data for SA-HRP

Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).

Application data for SA-HRP

Measure performance comparison between Acro (Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin) and other competitive products. With tight quality control measures, Acro demonstrates high sensitivity in this assay (as shown in the comparison data).

Application data for SA coated plate

SP-11-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

SP-11-E2

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

SP-11-E3

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituxima* with a linear range of 78-625 ng/mL (QC tested).

SP-11-E4

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimuma* with a linear range of 78-1250 ng/mL (QC tested).

Application data for SA magnetic beads

SMB-B01-E1

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads （Cat. No. SMB-B01） can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

Reference

  • Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.

MABSOL® Biotinylierte Proteine

  • SA- Biotin Systems
  • SA Series Products
  • Verification Data
  • Reference
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