Biotinylated Cynomolgus CD117 / c-kit Protein, His,Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
CD7-C82E4-25ug
$415.00
CD7-C82E4-200ug
$1705.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD117, SCFR, c-Kit, KIT

  • Source

    Biotinylated Cynomolgus CD117 Protein, His,Avitag (CD7-C82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 521 (Accession # XP_005555329.1).

    Predicted N-terminus: Gln 26

    Request for sequence
  • Molecular Characterization

    CD117 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 59.3 kDa. The protein migrates as 75-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD117 SDS-PAGE

    Biotinylated Cynomolgus CD117 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD117 SEC-MALS

    The purity of Biotinylated Cynomolgus CD117 Protein, His,Avitag (Cat. No. CD7-C82E4) is more than 90% and the molecular weight of this protein is around 75-105 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD117 ELISA

    Immobilized Human SCF, His Tag (Cat. No. SCF-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD117 Protein, His,Avitag (Cat. No. CD7-C82E4) with a linear range of 0.02-0.313 μg/mL (QC tested).

    Protocol
  •  CD117 ELISA

    Immobilized GMP Human SCF Protein (Cat. No. GMP-SCFH25) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD117 Protein, His,Avitag (Cat. No. CD7-C82E4) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

    Protocol

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バックグランド

Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Stem cell growth factor receptor

  • カテゴリ:

  • 市販の医薬品数:

    17 詳細

  • 治験医薬品数:

    40 詳細

  • 最新の開発ステージ:

    Approved

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