Biotinylated Human CD24 Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
CD4-H82E9-25ug
$360.00
CD4-H82E9-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD24, CD24A

  • Source

    Biotinylated Human CD24, His,Avitag (CD4-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Gly 59 (Accession # P25063-1).

    Predicted N-terminus: Ser 27

    Request for sequence
  • Molecular Characterization

    CD24 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 6.7 kDa. The protein migrates as 18-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD24 SDS-PAGE

    Biotinylated Human CD24, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CD24 ELISA

    Immobilized Biotinylated Human CD24, His,Avitag (Cat. No. CD4-H82E9) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-CD24 MAb (SN3) with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

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FAQ

  • Product

    What is the extinction coefficient of this product?

    The sequence of this product (including the target region and tags) contains no Trp, Tyr, or Cys residues; therefore, its extinction coefficient is 0.

Background

CD24 may have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity.

Recent Advances

 
Drug Development Progress
  • English Name:

    Signal transducer CD24

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Phase 2 Clinical

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