Biotinylated Human CD38 Protein, Avitag™

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製品番号/仕様
価格
数量
CD8-H8214-25ug
$390.00
CD8-H8214-200ug
$1490.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD38, T10, cADPr hydrolase 1

  • Source

    Biotinylated Human CD38 Protein, Avitag (CD8-H8214) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766.2).

    Predicted N-terminus: Val 43

    Request for sequence
  • Molecular Characterization

    CD38 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the C-terminus.

    The protein has a calculated MW of 32.8 kDa. The protein migrates as 43-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD38 SDS-PAGE

    Biotinylated Human CD38 Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     CD38 ELISA

    Immobilized Biotinylated Human CD38 Protein, Avitag (Cat. No. CD8-H8214) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD38 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     CD38 FACS

    2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human CD38 Protein, Avitag (Cat. No. CD8-H8214) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

    Protocol

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バックグランド

CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from D+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Lymphocyte differentiation antigen CD38

  • カテゴリ:

  • 市販の医薬品数:

    3 詳細

  • 治験医薬品数:

    47 詳細

  • 最新の開発ステージ:

    Approved

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