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Product Details
Synonyms
CDW52, HE5, CD52, CAMPATH-1 antigen
Source
Biotinylated Human CD52, His,Avitag (CD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ser 36 (Accession # P31358-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 13.5 kDa. The protein migrates as 13 kDa and 16-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human CD52, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Monoclonal Anti-Human CD52 Antibody, Human IgG1 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) with a linear range of 0.02-1 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Human CD52 MAb (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) with an affinity constant of 71.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) on SA Biosensor, can bind Anti-Human CD52 MAb (Human IgG1) with an affinity constant of 137 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CAMPATH-1 antigen
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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