Biotinylated Human GCGR / Glucagon receptor Protein, His,Avitag™ (MALS & SPR verified)

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Cat. No. / Size
Price
Qty
GCR-H82E3-25ug
$415.00
GCR-H82E3-200ug
$1705.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glucagon R, GCGR, Glucagon receptor

  • Source

    Biotinylated Human GCGR Protein, His,Avitag (GCR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Lys 136 (Accession # P47871-1).

    Predicted N-terminus: Ala 26

    Request for sequence
  • Molecular Characterization

    GCGR Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 16.6 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    GCGR SDS-PAGE

    Biotinylated Human GCGR Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    GCGR SEC-MALS

    The purity of Biotinylated Human GCGR Protein, His,Avitag (Cat. No. GCR-H82E3) is more than 85% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     GCGR SPR

    Biotinylated Human GCGR Protein, His,Avitag (Cat. No. GCR-H82E3) immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 48.5 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

Recent Advances

 
Drug Development Progress
  • English Name:

    Glucagon receptor

  • Category:

  • Approved Drugs:

    6 Details

  • Drugs in Clinical Trials:

    40 Details

  • Highest Development Stage:

    Approved

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