Biotinylated Human HLA-A*01:01&B2M&MAGEA3 (EVDPIGHLY) Complex Protein (Monomer, MALS verified)

Synonyms

HLA-A*0101 & B2M & MAGEA3 (EVDPIGHLY)

Source

Biotinylated Human HLA-A*01:01&B2M&MAGEA3 (EVDPIGHLY) Complex Protein (HL3-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*01:01) & Ile 21 - Met 119 (B2M) & EVDPIGHLY peptide (Accession # Q5SUL5 (HLA-A*01:01) & P61769-1 (B2M) & EVDPIGHLY).

Predicted N-terminus: Gly 25 & Glu

Molecular Characterization

Biotinylated Human HLA-A*01:01&B2M&MAGEA3 (EVDPIGHLY) Complex Protein is produced by co-expression of HLA-A*01:01 and B2M loaded with MAGEA3 peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.0 kDa and 13.8 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process