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Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein (Monomer, MALS verified)

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HL0-H82E8-25ug
$125.00
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HL0-H82E8-200ug
$700.00
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HL0-H82E8
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Product Details
Performance Data
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
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Synonyms

HLA-E*0103 & B2M & CMV UL40 (VMPPRTVIL)

Source

Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein (HL0-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMPPRTVIL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMPPRTVIL).

Predicted N-terminus: Gly 22 & Val

Molecular Characterization

Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.2 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.