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製品詳細
Synonyms
IL-11, Interleukin-11, AGIF, Oprelvekin, IL11
Source
Biotinylated Human IL-11, His,Avitag (IL1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20809-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Biotinylated Human IL-11, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) is more than 95% and the molecular weight of this protein is around 20-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) with a linear range of 0.4-25 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) on SA Biosensor, can bind Human IL-11 R alpha, His Tag (Cat. No. ILR-H52H5) with an affinity constant of 27 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Interleukin-11
- カテゴリ：
- 市販の医薬品数：
1 詳細
- 治験医薬品数：
4 詳細
- 最新の開発ステージ：
Approved
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