Biotinylated Mouse IL-12 R beta 1 / CD212 Protein, His,Avitag™

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ILB-M52E1-25ug
$360.00
In Stock - Delivery in 2-3 business days
ILB-M52E1-200ug
$1420.00
In Stock - Delivery in 2-3 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL-12 R beta 1, IL12RB1, CD212, IL12R, IL12RB, IL-12RB1

  • Source

    Biotinylated Mouse IL-12 R beta 1, His,Avitag (ILB-M52E1) is expressed from human 293 cells (HEK293). It contains AA Gln 20 - Ser 565 (Accession # Q60837-1).

    Predicted N-terminus: Gln 20

    Request for sequence
  • Molecular Characterization

    IL-12 R beta 1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 64.7 kDa. The protein migrates as 43-55 kDa and 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the SABA assay / HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-12 R beta 1 SDS-PAGE

    Biotinylated Mouse IL-12 R beta 1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     IL-12 R beta 1 ELISA

    Immobilized Biotinylated Mouse IL-12 R beta 1, His,Avitag (Cat. No. ILB-M52E1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol

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コメントを投稿

  • K***n2024-10-05
    いいね
    I’ve been using the IL12 receptor protein from Acro Biosystem in my antibody discovery research, and I’m highly satisfied with its quality. The protein exhibits excellent stability and bioactivity, which has been crucial in my experiments targeting immune responses. It’s been a reliable reagent, showing consistent results across various assays. I highly recommend this product for anyone involved in immunological research, particularly in therapeutic antibody development.

バックグランド

The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin-12 receptor

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    9 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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