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Product Details
Synonyms
IL-12 R beta 1, IL12RB1, CD212, IL12R, IL12RB, IL-12RB1
Source
Biotinylated Mouse IL-12 R beta 1, His,Avitag (ILB-M52E1) is expressed from human 293 cells (HEK293). It contains AA Gln 20 - Ser 565 (Accession # Q60837-1).
Predicted N-terminus: Gln 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 64.7 kDa. The protein migrates as 43-55 kDa and 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Mouse IL-12 R beta 1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Biotinylated Mouse IL-12 R beta 1, His,Avitag (Cat. No. ILB-M52E1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
K***n2024-10-05
LikesI’ve been using the IL12 receptor protein from Acro Biosystem in my antibody discovery research, and I’m highly satisfied with its quality. The protein exhibits excellent stability and bioactivity, which has been crucial in my experiments targeting immune responses. It’s been a reliable reagent, showing consistent results across various assays. I highly recommend this product for anyone involved in immunological research, particularly in therapeutic antibody development.
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-12 receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
9 Details
- Highest Development Stage：
Phase 2 Clinical
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