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Product Details
Synonyms
IL-22, IL-TIF, ZCYTO18
Source
Biotinylated Human IL-22, His,Avitag (IL2-H8247) is expressed from human 293 cells (HEK293). It contains AA Ala 34 - Ile 179 (Accession # Q9GZX6-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human IL-22, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well)on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Human IL-22 Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-22 R alpha 1, Fc Tag with a linear range of 0.6-78 ng/mL (Routinely tested).
Protocol
Immobilized Human IL-22BP Protein, Fc Tag (Cat. No. ILP-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 0.03-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-22BP Protein, His Tag (Cat. No. ILP-H52H3) with an affinity constant of 0.162 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
V***s2025-07-19
LikesWe purchased the "Biotinylated Human IL-22 Protein, His,Avitag™" from ACRO Biosystems. The quality of this product is great and behaves as expected in our experiments. The product support is great and you can find all the information you need on the website. The only suggestion I would have for this is to make it easier and faster to get protocols and data sheets without having to enter all you basic information to be able to download them. Other than that the only other "negative" review I would have is that there are hardly any discounts or promotions for this product. We bought many of the 25ug vials and, in hindsight, we should have ordered the 200ug to be a little more cost effective. Other than that, communication with a representative is fast and easy if needed.
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-22
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 3 Clinical
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