Biotinylated Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Synonyms

Integrin alpha 4 beta 7, ITGA4&ITGB7

Source

Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (IT7-H82W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).

Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 113.1 kDa (ITGA4) & 81.8 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 65-80 kDa, 95-130 kDa and 140-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process