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製品詳細
Synonyms
LRP5, LRP-5, LRP-7, LRP7, LR3
Source
Biotinylated Human LRP-5, His,Avitag (LR5-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 644 - Gln 1263 (Accession # O75197-1 ).
Predicted N-terminus: Glu 644
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 73.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
0.7-1.0 as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Biotinylated Human LRP-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) is more than 90% and the molecular weight of this protein is around 70-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human SOST, His Tag (Cat. No. HST-H5245) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.005-1.25 μg/mL (Routinely tested).
Protocol
Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.005-2.5 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human Dkk-1, His Tag (Cat. No. DK1-H5221) with an affinity constant of 93.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human SOST, His Tag (Cat. No. HST-H5245) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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最新の進展
医薬品開発の進捗- 英語名：
Low-density lipoprotein receptor-related protein 5
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
2 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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