Biotinylated Mouse Mesothelin / MSLN Protein, His,Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
MSN-M82E7-25ug
$260.00
MSN-M82E7-200ug
$1110.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    MSLN, Mesothelin, MPF

  • Source

    Biotinylated Mouse Mesothelin, His,Avitag (MSN-M82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 298 - Ser 600 (Accession # Q61468-1).

    Predicted N-terminus: Asp 298

    Request for sequence
  • Molecular Characterization

    Mesothelin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    After reconstitution, this product is stable after storage at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Mesothelin SDS-PAGE

    Biotinylated Mouse Mesothelin, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Mesothelin SEC-MALS

    The purity of Biotinylated Mouse Mesothelin, His,Avitag (Cat. No. MSN-M82E7) is more than 85% and the molecular weight of this protein is around 45-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Mesothelin ELISA

    Immobilized Biotinylated Mouse Mesothelin, His,Avitag (Cat. No. MSN-M82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 10-78 ng/mL (QC tested).

    Protocol

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バックグランド

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Mesothelin

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    55 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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