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Biotinylated Human NKG2D / CD314 Protein, Avitag™,Fc Tag (MALS verified)

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NKD-H82F4-25ug
$360.00
In Stock - Delivery in 1-2 business days
NKD-H82F4-200ug
$1,565.00
In Stock - Delivery in 1-2 business days
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NKD-H82F4
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

NKG2D, CD314, KLRK1, NK cell receptor D

Source

Biotinylated Human NKG2D, Avitag,Fc Tag (NKD-H82F4) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).

Predicted N-terminus: Gly

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

The protein has a calculated MW of 44.8 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Endotoxin

Less than 0.1 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.